Fiber-optic microsensors to measure backscattered light intensity in biofilms.
نویسندگان
چکیده
We have constructed tapered fiber-optic microsensors with a tip diameter of less than 10 microm to measure profiles of backscattered light in biofilms, which are thin layers of micro-organisms firmly attached to surfaces. The observed response agrees well with local effective diffusivity microelectrode measurements, with R(2) > 0.85. A strong relation between signal intensity and wavelength has been observed at 670 and 1320 nm. These sensors have the potential to replace local effective diffusivity microelectrodes for true in situ biofilm measurements.
منابع مشابه
Optical microsensors for analysis of microbial communities.
Fiber-optic microprobes connected to sensitive light meters are ideal tools to resolve the steep gradients of light intensity and spectral composition that prevail in aggregates and surface-associated microbial communities in sediments, biofilms, and microbial mats. They allow for a detailed mapping of light fields and enable insights to the complex optical properties of such highly light-scatt...
متن کاملMeasurement of intrinsic optical backscattering characteristics of cells using fiber-guided near infrared light
BACKGROUND Intrinsic optical signals (IOS), which reflect changes in transmittance and scattering light, have been applied to characterize the physiological conditions of target biological tissues. Backscattering approaches allow mounting of the source and detector on the same side of a sample which creates a more compact physical layout of device. This study presents a compact backscattering d...
متن کاملSize and shape determination of spheroidal scatterers using two-dimensional angle resolved scattering
We demonstrate accurate determination of the size and shape of spherical and spheroidal scatterers through inverse analysis of two-dimensional solid-angle and depth resolved backscattered light intensities. Intensity of scattered light is measured over a wide range of solid angles using a novel scanning fiber optic interferometer from both individual and ensembles of scatterers. T-matrix based ...
متن کاملFiber - Optic Sensing of Linear Thermal Expansion (RESEARCH NOTES)
The use of a LED fiber-optic sensor to measure displacement and linear thermal expansion is described. It has a sensitivity of about 0.6 mV/mm, a resolution of 1.25 mm, and a dynamic rang of 400 mm for displacement measurements. For thermal expansion, it shows a sensitivity of about 3.5 mV/C, and the experimental linear expansion values are in agreement with those calculated. The reported senso...
متن کاملImplementing a Practical Light Transmission System in order to Lighting an Office with Zero Energy Consumption
One of the recently considered applications of fiber optic, in their usage in building lighting systems. In this research, in order to reduce energy consumption, by transmission of sun light from the roof to the desired place (i.e. an office), the required standard luminance is produced. The main aims of this research are :1. Reduction of energy consumption.2. Making the place compatible with t...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Applied optics
دوره 39 19 شماره
صفحات -
تاریخ انتشار 2000